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宿主来源
Rabbit抗原名称
CGA/HCG-α分子别名
Glycoprotein hormones alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha免疫原
Recombinant Protein细胞定位
SecretedAccession
P01215克隆号
SDT-643-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS储存条件
12 months from date of receipt / reconstitution, 4°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:500
Human chorionic gonadotropin (HCG) is a glycoprotein hormone consisting of two noncovalently bonded subunits, alpha and beta [PMID: 68892], and is normally synthesized by placental trophoblasts during pregnancy. The alpha subunit of hCG is identical to the alpha subunit of 3 hormones synthesized by the anterior pituitary gland but the beta subunit of each of these hormones is unique and confers biological specificity. CGA is mainly used for the diagnosis and adjuvant diagnosis of placental trophoblast and germ cell tumors such as hyperemesis gravidarum and choriocarcinoma.
免疫印迹
WB result of CGA/HCG-α Rabbit mAb
Primary antibody: CGA/HCG-α Rabbit mAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 18 kDa
(This blot was developed with high sensitivity substrate)
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human spleen. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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