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Mouse抗原名称
MUC1分子别名
Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3 (CA 15-3), Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6 (KL-6), PEMT, Peanut-reactive urinary mucin (PUM), Polymorphic epithelial mucin (PEM), Tumor-associated epithelial membrane antigen (EMA), Tumor-associated mucin免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P15941克隆号
SDT-777-37抗体类型
Mouse mAb抗体同种型
IgG1,k应用
ICFCM, IHC-P, ICC, WB, IP, IF反应种属 ?
Hu, Rt预测反应种属
(反应种属缩写表)Pr纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:200 |
| IHC-P | 1:500-1:2000 |
| ICC | 1:500 |
| ICFCM | 1:2000 |
| IF | 1:500 |
MUC1 is a transmembrane protein normally expressed in glandular or luminal epithelial cells and hematopoietic cells and absent in skin epithelium and mesenchymal cells. Apical surface of almost all glandular and ductal epithelial cells, including respiratory, gastrointestinal, urogenital, hepatobiliary tracts, sebaceous and salivary glands. MUC1 is expressed in the central cells of pancreatic acini, intercalary duct epithelium, and interlobular duct epithelium, but not in the acinar epithelium, main pancreatic duct epithelium, and pancreatic islet epithelium. Overexpression is common in malignancies and leads to tumorigenesis. Increased expression is associated with poor prognosis of most carcinomas, including those involving breast, cervical, pancreatic, colorectal.
免疫印迹
WB result of MUC1 Mouse mAb
Primary antibody: MUC1 Mouse mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 122 kDa
Observed MW: 24 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HCT116 (Human colorectal carcinoma epithelial cell, left) / T-47D (Human ductal breast epithelial tumor epithelial cell, Right) labelling MUC1 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: HCT116
免疫沉淀
MUC1 Mouse mAb at 1/200 dilution (1 µg) immunoprecipitating MUC1 in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using MUC1 Mouse mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/2000 dilution.
Lane 1: T-47D whole cell lysate 20 µg (Input)
Lane 2: MUC1 Mouse mAb IP in T-47D whole cell lysate
Lane 3: Mouse monoclonal IgG1 IP in T-47D whole cell lysate
Predicted MW: 122 kDa
Observed MW: 24 kDa
（This blot was developed with high sensitivity substrate）
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-MUC1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-MUC1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in T-47D cells. Anti-MUC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in HCT116 cells. Anti-MUC1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
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IF shows positive staining in paraffin-embedded human kidney. Anti-MUC1 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor®594) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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IF shows positive staining in paraffin-embedded human stomach. Anti-MUC1 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor®594) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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IF shows positive staining in paraffin-embedded human tonsil. Anti-MUC1 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor®594) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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