S-RMab^® Cadherin 17 Recombinant Rabbit mAb (SDT-736-23)

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货号： S0B2291

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Cadherin 17
分子别名

LI cadherin, Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, CDH17
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

Q12864
克隆号

SDT-736-23
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

IHC-P, WB
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500-1:2000

背景介绍

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. In normal tissues, LI cadherin antibodies are labeled on epithelial cells of the gastrointestinal and pancreatic tracts, but not on epithelial cells of the kidneys, liver, and other tissues. In tumors, it is expressed in digestive system adenocarcinoma, including cancer. It is a sensitive biomarker for distinguishing gastrointestinal metaplasia from well differentiated adenocarcinoma.

免疫印迹
- WB result of Cadherin 17 Rabbit mAb
  Primary antibody: Cadherin 17 Rabbit mAb at 1/1000 dilution
  Lane 1: PANC-1 whole cell lysate 20 µg
  Lane 2: LoVo whole cell lysate 20 µg
  Negative control: PANC-1 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 92 kDa
  Observed MW: 120 kDa
  Exposure time: 2 s (This blot was developed with high sensitivity substrate)
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti- Cadherin 17 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.