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Rabbit抗原名称
Claudin 5分子别名
Transmembrane protein deleted in VCFS (TMDVCF), CLDN5, AWAL, TMVCF细胞定位
Cell membraneAccession
O00501克隆号
SDT-R322抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS储存条件
12 months from date of receipt / reconstitution, 4°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
Claudin-5 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction (TJ) strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-5 is an endothelial cell-specific component of TJ strands. It has been used as a marker for endothelial lesions. Claudin-5 is also found in bronchial and lung epithelial cells. In tumors, Claudn-5 expression has been found in lung adenocarcinoma and squamous carcinoma. Strong membranous expression of claudin5 and claudin7 differentiates pancreatic solid papillary neoplasm from pancreatoblastoma, acinar cell and endocrine tumors.
免疫印迹
WB result of Claudin 5 Rabbit mAb
Primary antibody: Claudin 5 Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDaWB result of Claudin 5 Rabbit mAb
Primary antibody: Claudin 5 Rabbit mAb at 1/1000 dilution
Lane 1: rat lung unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human angiosarcoma. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Claudin 5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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