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宿主来源
Rabbit抗原名称
Langerin分子别名
C-type lectin domain family 4 member K, CD207, CLEC4K免疫原
Synthetic Peptide细胞定位
MembraneAccession
Q9UJ71克隆号
SDT-450-47抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P反应种属 ?
Hu纯化方式
Protein A浓度
0.25 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:1000 |
Langerin (CD207) is a type II transmembrane C-type lectin which is encoded by the CD207 gene in humans. Langerin is known to be associated with the formation of Birbeck granules in Langerhans cells (LCs). LCs are immune cells closely related to macrophages, but by their function, they are more like conventional dendritic cells (cDCs). Langerin is a highly selective marker for Langerhans cells and the lesional cells of Langerhans cell histiocytosis.
免疫组化
IHC shows positive staining in paraffin-embedded human esophagus. Anti-Langerin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skin. Anti-Langerin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Langerin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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