FSHB Recombinant Rabbit mAb (SDT-R287)

Follitropin subunit beta,Follicle-stimulating hormone beta subunit,Follitropin beta chain

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B2272

规格

25μl100μl500μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

FSHB
分子别名

Follitropin subunit beta, Follicle-stimulating hormone beta subunit, Follitropin beta chain
细胞定位

Secreted
Accession

P01225
克隆号

SDT-R287
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Rt
纯化方式

Protein A
浓度

0.125 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

IF ?
稀释度

应用稀释度

IHC 1:1000
IF 1:500

应用	稀释度
IHC	1:1000
IF	1:500

背景介绍

Follicle-stimulating hormone beta subunit, together with the alpha chain CGA constitutes Follicle-stimulating hormone (FSH). FSH is involved in follicle development and spermatogenesis in reproductive organs. In pathology, it can be used to auxiliary the typing of pituitary tumours.

免疫组化
- IHC shows positive staining in paraffin-embedded human pituitary. Anti-FSHB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-FSHB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-FSHB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat pituitary. Anti-FSHB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-FSHB antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human pituitary. Anti-FSHB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.