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Rabbit抗原名称
CK-13分子别名
Cytokeratin-13, Keratin-13, K13, KRT13, CK 13, Cytokeratin 13, Keratin 13细胞定位
CytoskeletonAccession
P13646克隆号
SDT-R282抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC | 1:1000 |
Cytokeratin 13 (CK13), also known as keratin 13 (KRT13), is a type I acidic polymer metamorphine. It forms a heterotetramer with type I CK4 and forms an intermediate wire, mainly to shape the cell skeleton of specific epithelial cells. It is mainly expressed in suprabasal layers of noncornified stratified epithelium, including squamous (nonkeratinized) epithelia of cervix, esophagus, larynx, oral cavity, tonsils, urothelium and respiratory type epithelium. It is a marker of mature but nonkeratinized squamous epithelium.
免疫印迹
WB result of CK-13 Rabbit mAb
Primary antibody: CK13 Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 51 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophagus. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-CK-13 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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