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宿主来源
Rabbit抗原名称
CA125分子别名
Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, MUC16, Cancer antigen 125免疫原
Synthetic Peptide细胞定位
Extracellular space, Secreted, Cell membraneAccession
Q8WXI7克隆号
SDT-555-41抗体类型
Recombinant mAb应用
IHC-P, WB反应种属 ?
Hu纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC | 1:500 |
Mucin-16 (MUC-16), also known as ovarian cancer associated tumor marker CA125, is a protein encoded by the MUC16 gene. MUC-16 is a member of the glycoprotein family of mucins. MUC-16 has been applied as a tumor marker or biomarker, which may be elevated in the blood of certain types of cancer patients, especially those with cancer or other benign diseases. Detecting CA-125 blood levels is considered useful for treating ovarian cancer. Although this test can provide useful information for women who are known to have cancer, due to the uncertain correlation between CA-125 levels and cancer, the CA-125 test has not been found to be useful as a screening method. In addition to ovarian cancer, CA-125 also increases in patients with endometrial cancer, fallopian tube cancer, lung cancer, breast cancer, and gastrointestinal cancer. Pregnant women may also experience this condition in their bodies. Due to the fact that serum levels can be increased under various circumstances, CA-125 is not used for cancer detection, but it is typically used to monitor ovarian cancer patients' response to chemotherapy, recurrence, and disease progression.
免疫印迹
WB result of CA125 Rabbit mAb
Primary antibody: CA125 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: OVCAR3 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate;
SK-OV-3 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 1519 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-CA125 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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