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Rabbit抗原名称
PCNA分子别名
Proliferating cell nuclear antigen, Cyclin免疫原
Synthetic Peptide细胞定位
NucleusAccession
P12004克隆号
SDT-297-116抗体类型
Recombinant mAb反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Av, Bv, Hm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM ,IHC-P ? ,ICC ? ,WB ,IP稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC 1:2000 ICC 1:500 Hu ICFCM 1:50 Hu
Proliferating cell nuclear antigen (PCNA) is a type of DNA clamp that acts as a DNA polymerase in eukaryotic cells δ. The processing factor of is crucial for replication. PCNA is a homologous trimer that achieves its processability by surrounding DNA, where it serves as a scaffold for recruiting proteins involved in DNA replication, DNA repair, chromatin remodeling, and epigenetics. It is expressed in most tumors as a proliferative antigen.
免疫印迹
WB result of PCNA Rabbit mAb
Primary antibody: PCNA Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDaWB result of PCNA Rabbit mAb
Primary antibody: PCNA Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Lane 3: NIH/3T3 whole cell lysate 20 µg
Lane 4: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDaWB result of PCNA Rabbit mAb
Primary antibody: PCNA Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 33 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PCNA antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
PCNA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PCNA in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using PCNA Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: PCNA Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 29 kDa
Observed MW: 33 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PCAN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-PCNA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
引用文献(1)
- 基于Notch1/HES1通路探讨淫羊藿苷对衰老小鼠睾丸支持细胞胶质细胞源性神经营养因子表达的影响
DUAN Haili,CHEN Yufeng，ZHANG Changcheng，FU Guoqing，MAO Runmin，QU Yanan，GAO Zihui，WU Jie，ZHAO Haixia1
Chinese Traditional and Herbal Drugs. 2025 Sep 25 .
影响因子： 5.342
货号：S0B2253产品名称：PCNA Recombinant Rabbit mAb (SDT-297-116)
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