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Glutamine Synthetase (GS) Recombinant Rabbit mAb,PBS Only (SDT-549-25)

Glutamate--ammonia ligase,Palmitoyltransferase GLUL,GLUL,GLNS

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货号 S0B2249P
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Glutamine Synthetase (GS)

  • 分子别名

    Glutamate--ammonia ligase, Palmitoyltransferase GLUL, GLUL, GLNS

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm

  • Accession

    P15104

  • 克隆号

    SDT-549-25

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS

  • 储存条件

    12 months from date of receipt / reconstitution, 4 °C as supplied

  • 应用

    IHC-P ? ,
    WB

  • 稀释度

    应用 稀释度
    WB 1:1000-1:5000
    IHC 1:500-1:1000
背景介绍

  • Glutamine synthetase (GS) is a 42 kDa protein. GS converts glutamate and ammonia into glutamine using adenosine triphosphate (ATP). GS exerts critical biological functions in liver, kidney, skeletal muscle, and brain. In cancer, GS plays a pivotal role in restructuring the cell metabolism in order to enable continued proliferation and survival of malignant cells in poorly vascularized and nutrient-deprived environments. GS usually combines with Glypican and HSP70 for differentiated diagnosis of liver hypoperia nodules and hepatocellular carcinoma.

  • 免疫印迹

    • WB result of Glutamine Synthetase (GS) Rabbit mAb
      Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/1000 dilution
      Lane 1: SK-MEL-28 whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Negative control: SK-MEL-28 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

    • WB result of Glutamine Synthetase (GS) Rabbit mAb
      Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/5000 dilution
      Lane 1: mouse brain lysate 5 µg
      Lane 2: mouse liver lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

    • WB result of Glutamine Synthetase (GS) Rabbit mAb
      Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/5000 dilution
      Lane 1: rat brain lysate 5 µg
      Lane 2: rat liver lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human breast. Anti- Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human glioma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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