S-RMab^® PAX5 Recombinant Rabbit mAb (SDT-R202)

浏览次数：13

货号： S0B2226

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

PAX5
分子别名

Paired box protein Pax-5, B-cell-specific transcription factor (BSAP), PAX-5, PAX 5
细胞定位

Nucleus
Accession

Q02548
克隆号

SDT-R202
抗体类型

Rabbit mAb
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IP	1:50
IHC	1:500
ICC	1:500
ICFCM	1:50

背景介绍

The transcription factor Pax5 is essential for commitment of lymphoid progenitors to the B lymphocyte lineage. Pax5 fulfils a dual role by repressing B lineage 'inappropriate' genes and simultaneously activating B lineage–specific genes. This transcriptional reprogramming restricts the broad signaling capacity of uncommitted progenitors to the B cell pathway, regulates cell adhesion and migration, induces VH-DJH recombination, facilitates (pre-)B cell receptor signaling and promotes development to the mature B cell stage. Conditional Pax5 inactivation in early and late B lymphocytes revealed an essential role for Pax5 in controlling the identity and function of B cells throughout B lymphopoiesis. PAX5 has also been implicated in human B cell malignancies, as it is deregulated by chromosomal translocations in a subset of acute lymphoblastic leukemias and non-Hodgkin lymphomas.

免疫印迹
- WB result of PAX5 Rabbit mAb Primary antibody: PAX5 Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: Raji whole cell lysate 20 µg Lane 3: Ramos whole cell lysate 20 µg Lane 4: Daudi whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa Observed MW: 45 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, left) / Raji (Human Burkitt's lymphoma B lymphocyte, right) cells labelling PAX5 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
  Negative control: Jurkat
免疫沉淀
- PAX5 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PAX5 in 0.4mg Ramos whole cell lysate. Western blot was performed on the immunoprecipitate using PAX5 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: Ramos whole cell lysate 20µg(input) Lane 2: PAX5 Rabbit mAb IP in Ramos whole cell lysate Lane 3: Rabbit monoclonal IgG IP in Ramos whole cell lysate Predicted MW: 42 kDa Observed MW: 45 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human NK/T-cell lymphoma. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti-PAX5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Ramos cells. Anti-PAX5 antibody was used
  at 1/500 dilution (Green) and incubated overnight at 4°C.
  Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
  was used as secondary antibody at 1/1000 dilution.
  The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100.
  Nuclei were counterstained with DAPI.
- Negative control: ICC shows negative staining in Jurkat cells.
  Anti-PAX5 antibody was used at 1/500 dilution and incubated overnight at 4°C.
  Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
  was used as secondary antibody at 1/1000 dilution.
  The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100.
  Nuclei were counterstained with DAPI.