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宿主来源
Rabbit抗原名称
Mesothelin分子别名
CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, MSLN免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
Q13421克隆号
SDT-347-15抗体类型
Rabbit mAb应用
IHC-P, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS储存条件
12 months from date of receipt / reconstitution, 4 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IP | 1:50 |
| IHC | 1:1000 |
Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein normally expressed in mesothelial cells that line the pleura, peritoneum, and pericardium. The MSLN gene encodes a precursor protein of 71 kDa that is processed to a 31 kDa shed protein called MPF (megakaryocyte potentiating factor) and a 40 kDa membrane bound protein, mesothelin. Mesothelin is reported to be highly expressed in several types of malignant tumors, such as malignant mesothelioma, ovarian cancer, pancreatic adenocarcinoma, and lung adenocarcinoma.
免疫印迹
WB result of Mesothelin Rabbit mAb Primary antibody: Mesothelin Rabbit mAb at 1/1000 dilution Lane 1: PC-3 whole cell lysate 20 µg Lane 2: HeLa whole cell lysate 20 µg Lane 3: SK-OV-3 whole cell lysate 20 µg Lane 4: OVCAR-3 whole cell lysate 20 µg Negative control: PC-3 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa Observed MW: 46~48, 69 kDa
免疫沉淀
Mesothelin Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Mesothelin in 0.4mg HeLa whole cell lysate. Western blot was performed on the immunoprecipitate using Mesothelin Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: HeLa whole cell lysate 20 µg (Input) Lane 2: Mesothelin Rabbit mAb IP in HeLa whole cell lysate Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate Predicted MW: 69 kDa Observed MW: 46~48,69 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial carcinoma. Anti-Mesothelin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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