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p40(BC28) Recombinant Rabbit mAb (SDT-166-36)

Tumor protein 63,p63,Chronic ulcerative stomatitis protein (CUSP),Keratinocyte transcription factor KET,Transformation-related protein 63 (TP63),Tumor protein p73-like (p73L),p51,TP63,KET,P73H,P73L,TP73L

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B2212

规格

25μl100μl500μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

p40
分子别名

Tumor protein 63; p63; Chronic ulcerative stomatitis protein (CUSP); Keratinocyte transcription factor KET; Transformation-related protein 63 (TP63); Tumor protein p73-like (p73L); p51; TP63; KET; P73H; P73L; TP73L
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q9H3D4-4
克隆号

SDT-166-36
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC-P 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC-P	1:500

背景介绍

p63 (TAp63) is closely related to p40 (ΔNp63) as both proteins represent isoforms of the p63 gene with distinct molecular functions. While “full length” p63 (TAp63) activates p53 target genes such as p21 or BAX, the shorter transcript p40 (ΔNp63) inhibits activation of p53 and “full length” p63. Among carcinomas, p40 has approximately the same sensitivity as p63 but a higher specificity, as the TAp63 isoform is expressed more widespread in eg., adenocarcinomas. Moreover, p63 occur in lymphomas that are p40 negative.

免疫印迹
- WB result of p40 Rabbit mAb Primary antibody: p40 Rabbit mAb at 1/1000 dilution Lane 1: MCF7 whole cell lysate 20 µg Lane 2: A431 whole cell lysate 20 µg Lane 3: HaCaT whole cell lysate 20 µg Negative control: MCF7 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa Observed MW: 51, 75 kDa
免疫沉淀
- p40 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating p40 in 0.4 mg HaCaT whole cell lysate.
  Western blot was performed on the immunoprecipitate using p40 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HaCaT whole cell lysate 10 µg (Input)
  Lane 2: p40 Rabbit mAb IP in HaCaT whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HaCaT whole cell lysate
  Predicted MW: 51 kDa
  Observed MW: 51, 75 kDa
  （This blot was developed with high sensitivity substrate）
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-p40 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.