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Rabbit抗原名称
NY-ESO-1分子别名
Cancer/testis antigen 1, CTAG1B, CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, LAGE-2, CTAG1A, LAGE2免疫原
N/A细胞定位
CytoplasmAccession
P78358克隆号
SDT-R154抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
IHC-P ? ,WB稀释度
应用 稀释度 WB 1:1000 IHC-P 1:500
New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies [PMID: 25412843]. It is a protein consisting of 180 amino acids, and its gene is located in the Xq28 region of the X chromosome. As a member of the cancer testis antigen (CTA) family, NY-ESO-1 has been shown to be expressed in spermatogonia, primary spermatocytes, oogonia, and placenta and in a variety of cancers, such as melanoma, ovarian cancer, cervical cancer [PMID: 28025978, PMID: 25917631].
免疫印迹
WB result of NY-ESO-1 Rabbit mAb
Primary antibody: NY-ESO-1 Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: TT whole cell lysate 20 µg
Lane 4: A375 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18kDa
Observed MW: 20kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human seminoma. Anti-NY-ESO-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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