N-Cadherin Recombinant Rabbit mAb,PBS Only (SDT-329-12)

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货号： S0B2199P

Datasheet COA

价格：询价
规格：
1mg
数量：

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

N-Cadherin
分子别名

Cadherin-2, CDw325, Neural cadherin, CDH2, CDHN, NCAD
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P19022
克隆号

SDT-329-12
抗体类型

Recombinant mAb
应用

IHC-P, WB
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bovine, Xenopus, Monkey, Chimpanzee, Bat, Marmoset
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt, 4°C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:500

背景介绍

The cell adhesion molecule (CAM), N-cadherin, has emerged as an important oncology therapeutic target. N-cadherin is a transmembrane glycoprotein mediating the formation and structural integrity of blood vessels. Its expression has also been documented in numerous types of poorly differentiated tumours [PMID: 25533096].

免疫印迹
- WB result of N-Cadherin Rabbit mAb
  Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: PC-3 whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Negative control: MCF7 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 100kDa
  Observed MW: 110~140kDa
- WB result of N-Cadherin Rabbit mAb
  Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 100kDa
  Observed MW: 140kDa
- WB result of N-Cadherin Rabbit mAb
  Primary antibody: N-Cadherin Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Lane 2: rat heart lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 100kDa
  Observed MW: 110~140kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative tissue: IHC shows negative staining in paraffin-embedded human chromophobe renal carcinoma. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-N-Cadherin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.