S-RMab^® PD-1 Recombinant Mouse mAb (SDT-R143)

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货号： S0B2187

Datasheet COA

价格： ￥600
规格：
25ul100μl500μl1ml
数量：

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产品规格

宿主来源

Mouse
抗原名称

PD-1
分子别名

Programmed cell death protein 1, hPD-1, CD279
免疫原

N/A
细胞定位

Cell membrane
Accession

Q15116
克隆号

SDT-R143
抗体类型

Recombinant mAb
抗体同种型

IgG1
应用

ICFCM, IHC-P, ICC, WB, IP
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标签

N/A
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:200-1:500
ICFCM	1:200
ICC	1:100
WB	1:1000
IP	1:200

背景介绍

Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).

免疫印迹
- WB result of PD-1 Mouse mAb
  Primary antibody: PD-1 Mouse mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: MOLT-4 whole cell lysate 20 µg
  Lane 3: MOLT-4 treated with Ionomycin (500ng/ml 24 hr) and PMA (10ng/ml 24 hr) whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate
  Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 32 kDa
  Observed MW: 38~70 kDa
流式分析
- Flow cytometric analysis of Molt-4 (Human cervix adenocarcinoma epithelial cell) cells, treated with 10ng/ml PMA and 500ng/ml Ionomycin for 24h (Red) or untreated (Green), labeling PD-1 at 1/200 dilution (1 μg) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
- PD-1 Mouse mAb at 1/200 dilution (1 µg) immunoprecipitating PD-1 in 0.35 mg MOLT-4 treated with Ionomycin (500ng/ml 24 hr) and PMA (10ng/ml 24 hr) whole cell lysate.
  Western blot was performed on the immunoprecipitate using PD-1 Mouse mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/2000 dilution.
  Lane 1: MOLT-4 treated with Ionomycin (500ng/ml 24 hr) and PMA (10ng/ml 24 hr) whole cell lysate 20 µg (Input)
  Lane 2: PD-1 Mouse mAb IP in MOLT-4 treated with Ionomycin (500ng/ml 24 hr) and PMA (10ng/ml 24 hr) whole cell lysate
  Lane 3: Mouse monoclonal IgG1 IP in MOLT-4 treated with Ionomycin (500ng/ml 24 hr) and PMA (10ng/ml 24 hr) whole cell lysate
  Predicted MW: 32 kDa
  Observed MW: 38~70 kDa
  （This blot was developed with high sensitivity substrate）
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-PD-1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-PD-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PD-1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in MOLT-4 cells treated with Ionomycin (500ng/ml,24h) + PMA (10ng/ml,24h). Anti-PD-1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control：ICC shows negative staining in MOLT-4 cells untreated with Ionomycin (500ng/ml,24h) + PMA (10ng/ml,24h). Anti-PD-1 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).