Myosin-11/SMMHC Recombinant Rabbit mAb (SDT-188-112)

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货号： S0B2173

Datasheet COA

价格： ￥600
规格：
25μl100 μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Myosin-11/SMMHC
分子别名

smooth muscle myosin heavy chain, HSM-V, SMMS-1, SM-MYOSIN-H, Myosin heavy chain 11, MYH11
免疫原

Synthetic Peptide
细胞定位

Melanosome
Accession

P35749
克隆号

SDT-188-112
抗体类型

Recombinant mAb
应用

IHC-P, WB, IF
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Rb
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000-1:25000
IHC-P	1:1000
IF	1:125

背景介绍

SMMHC is a structural component of the smooth muscle myosin molecule and is a specific marker of “terminal” smooth muscle differentiation [PMID: 10721417, PMID: 3882826]. SMMHC is composed of at least two isoforms: SM1 (204 kDa) and SM2 (200 kDa), both of which are encoded by a single gene [PMID: 9702855, PMID: 10998642]. The SM1 isoform is expressed in the MEC of normal mammary glands, fibrocystic diseases, and in myoepithelial derived tumours of the breast [PMID: 11493962, PMID: 9702855]. Furthermore, studies have documented that antibodies to SMMHC and calponin, both markers of terminal smooth muscle differentiation, are more specific for breast MEC than are other more commonly used antibodies, such as those that recognise SMA [PMID: 11493962].

免疫印迹
- WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: mouse skeletal muscle lysate 5 µg Lane 2: mouse bladder lysate 5 µg Negative control: mouse skeletal muscle lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s
- WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/1000 dilution Lane 1: mouse skeletal muscle lysate 20 µg Lane 2: mouse prostate lysate 20 µg Negative control: mouse skeletal muscle lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 60s
- WB result of Myosin-11 Rabbit mAb Primary antibody: Myosin-11 Rabbit mAb at 1/25000 dilution Lane 1: rat skeletal muscle lysate 5 µg Lane 2: rat bladder lysate 5 µg Negative control: rat skeletal muscle lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 227kDa Observed MW: 250kDa Exposure time: 30s
免疫组化
- IHC shows positive staining in paraffin-embedded human breast. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human leiomyosarcoma. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-Myosin-11 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IF shows positive staining in paraffin-embedded human colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse colon. Anti-Myosin-11 antibody was used at 1/125 dilution (Green). Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.