FAP Recombinant Rabbit mAb (SDT-R132)
FAP,SEPR,Prolyl endopeptidase FAP,170 kDa melanoma membrane-bound gelatinase,Dipeptidyl peptidase FAP,Fibroblast activation protein alpha,FAPalpha,Gelatine degradation protease FAP,Integral membrane serine protease,Post-proline cleaving enzyme,Serine integral membrane protease,SIMP,Surface-expressed protease,Seprase
货号： S0B2166
- 价格： ￥600
- 规格：
- 数量：
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宿主来源
Rabbit抗原名称
FAP分子别名
Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, SIMP, Seprase免疫原
N/A细胞定位
Cell membrane, Cell SurfaceAccession
Q12884克隆号
SDT-R132应用
IHC-P, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:500 |
Fibroblast activation protein (FAP) is best known for its heightened expression in tumour stroma. This atypical serine protease has both dipeptidyl peptidase and endopeptidase activities, cleaving substrates at a post-proline bond. FAP expression is difficult to detect in non-diseased adult organs, but is greatly upregulated in sites of tissue remodelling, which include liver fibrosis, lung fibrosis, atherosclerosis, arthritis, tumours and embryonic tissues. Due to its restricted expression pattern and dual enzymatic activities, FAP is emerging as a unique therapeutic target [PMID: 24470260].
免疫印迹
WB result of FAP Rabbit mAb Primary antibody: FAP Rabbit mAb at 1/1000 dilution Lane 1: SK-MEL-28 whole cell lysate 20 µg Lane 2: HFF whole cell lysate 20 µg Low expression control: SK-MEL-28 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 87 kDa Observed MW: 95 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-FAP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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