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宿主来源
Rabbit抗原名称
CD47分子别名
Leukocyte surface antigen CD47, IAP, MER6细胞定位
Cell membraneAccession
Q08722克隆号
SDT-R121抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
IHC-P ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:200
The transmembrane protein, CD47, is recognized as an important innate immune checkpoint, and CD47-targeted drugs have been in development with the aim of inhibiting the interaction between CD47 and the regulatory glycoprotein SIRPα, for antitumor immunotherapy. Further, CD47 mediates other essential functions such as cell proliferation, caspase-independent cell death (CICD), angiogenesis and other integrin-activation-dependent cell phenotypic responses when bound to thrombospondin-1 (TSP-1) or other ligands. Mounting strategies that target CD47 have been developed in pre-clinical and clinical trials, including antibodies, small molecules, siRNAs, and peptides, and some of them have shown great promise in cancer treatment [PMID: 35931392].
免疫印迹
- WB result of CD47 Rabbit mAb Primary antibody: CD47 Rabbit mAb at 1/1000 dilution Lane 1: HepG2 whole cell lysate 20 µg Lane 2: NIH:OVCAR-3 whole cell lysate 20 µg Negative control: HepG2 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 40~50 kDa Exposure time: 60s
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD47 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-CD47 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD47 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian carcinoma. Anti-CD47 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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