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宿主来源
Rabbit抗原名称
CD278/ICOS分子别名
Inducible T-cell costimulator, AILIM细胞定位
Secreted, Cell membraneAccession
Q9Y6W8克隆号
SDT-R104抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
IHC-P ?
稀释度
应用 稀释度 IHC-P 1:500
Inducible T cell costimulatory factor (ICOS, cluster of differentiation, CD278) is an activated costimulatory immune checkpoint expressed on T cells. Its ligand ICOSL was expressed in antigen-presenting cells and somatic cells (including tumor cells in the tumor microenvironment) [PMID: 28713380, PMID: 12800259]. As one of the most common targeted immune checkpoints, ICOS is considered a possible way to develop new immunotherapy [PMID: 29544515]. Some clinical studies shown that blocking ICOS alone can inhibit tumor growth and proliferation, as well as TIGIT, CTLA4, and PD-1 [PMID: 30232519, PMID: 28803728].
免疫组化
IHC shows positive staining in paraffin-embedded human thymus. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CD278/ICOS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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