S-RMab^® SMAD4 Recombinant Rabbit mAb (SDT-R114)

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货号： S0B2142

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Smad4
分子别名

Smad4, hSMAD4, DPC4
免疫原

N/A
细胞定位

Cytoplasm, Nucleus
Accession

Q13485
克隆号

SDT-R114
抗体类型

Rabbit mAb
应用

IHC-P, WB
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.25 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:1000

背景介绍

SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members.

免疫印迹
- WB result of SMAD4 Rabbit mAb
  Primary antibody: SMAD4 Rabbit mAb at 1/500 dilution
  Lane 1: HT-29 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: HCT 116 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Negative control: HT-29 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 65 kDa
- WB result of SMAD4 Rabbit mAb
  Primary antibody: SMAD4 Rabbit mAb at 1/500 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 65 kDa
- WB result of SMAD4 Rabbit mAb
  Primary antibody: SMAD4 Rabbit mAb at 1/500 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 65 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows partial loss of SMAD4 expression in paraffin-embedded human colon cancer. Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer (Loss of SMAD4 expression in tumors but nuclear expression in stromal cells). Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SMAD4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.