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Rabbit抗原名称
Keratin 17分子别名
Cytokeratin-17, CK-17免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q04695克隆号
SDT-011-43抗体类型
Rabbit mAb应用
IHC-P, ICC, WB反应种属 ?
Hu, Ms预测反应种属
(反应种属缩写表)Gor, Pr, Cz, Or, Bv, Sh, Rt, Ct, Rb, Dg, Hdg, Pr, Bb, Hr纯化方式
Protein A浓度
0.125 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| IHC-P | 1:1000 |
| WB | 1:250-1:1500 |
| ICC | 1:125 |
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. CK17 maybe an excellent marker for the identification of squamous cell carcinomas in various tissues including the cervix, lung and oral cavity.
免疫印迹
WB result of Keratin 17 Rabbit mAb
Primary antibody: Keratin 17 Rabbit mAb at 1/1250 dilution
Lane 1: MOLT-4 whole cell lysate 5 µg
Lane 2: HeLa whole cell lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
Exposure time: 7.5s
WB result of Keratin 17 Rabbit mAb
Primary antibody: Keratin 17 Rabbit mAb at 1/250 dilution
Lane 1: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
Exposure time: 30s
WB result of Keratin 17 Rabbit mAb
Primary antibody: Keratin 17 Rabbit mAb at 1/250 dilution
Lane 1: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
Exposure time: 30s
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical carcinoma. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-Keratin 17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Keratin 17 antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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