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宿主来源
Rabbit抗原名称
Mammaglobin A分子别名
Mammaglobin-1细胞定位
SecretedAccession
Q13296克隆号
SDT-R065抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
IHC-P ?
稀释度
应用 稀释度 IHC-P 1:250
Mammaglobin-A is a novel breast cancer-associated antigen that was initially identified using a differential screening approach. Subsequently, it has been demonstrated that MGBA has several unique properties which make it a promising target for immune therapy. Gene expression analysis of over 20 different human tissues demonstrated that MGBA is expressed almost exclusively in normal breast epithelium and breast cancer. MGBA is over expressed in up to 80% of primary and metastatic breast cancers.
免疫组化
IHC shows positive staining in paraffin-embedded human breast carcinoma. Anti-Mammaglobin A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast carcinoma. Anti-Mammaglobin A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast. Anti-Mammaglobin A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical carcinoma. Anti-Mammaglobin A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Mammaglobin A antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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