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宿主来源
Rabbit抗原名称
CDH17分子别名
Cadherin-17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin (LI-cadherin)免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q12864克隆号
SDT-3276-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:1000-1:2000 Hu
CDH17 (Cadherin-17, also known as LI-cadherin or HPT-1) is a unique member of the 7D-cadherin subclass within the cadherin superfamily. Structurally, it features an extracellular region containing seven cadherin repeats and a very short cytoplasmic domain (only 18–22 amino acids), which prevents it from binding to catenins like classical cadherins. Under normal physiological conditions, CDH17 is mainly expressed in the embryonic liver and the adult small intestine and colon, where it functions as a cell adhesion molecule and peptide transporter. However, it is aberrantly overexpressed in various gastrointestinal malignancies, including hepatocellular carcinoma, gastric cancer, colorectal cancer, and pancreatic cancer, and is closely associated with tumor invasion, metastasis, and poor prognosis. Studies have shown that CDH17 promotes tumor cell proliferation, survival, migration, and invasion by activating key signaling pathways such as Wnt, NF-κB/MAPK, and Akt. Due to its highly specific expression pattern in advanced gastrointestinal cancers, CDH17 has been recognized as a promising novel diagnostic biomarker and a highly potential target for cancer therapy.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDH17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded KATOIII cell pellets. Anti-CDH17 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded HCT15 cell pellets. Anti-CDH17 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CDH17 in tumor tissues.
Expression of CDH17 in human tissues.
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