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宿主来源
Rabbit分子别名
Catenin delta-1,p120 catenin (p120(ctn))免疫原
Recombinant Protein细胞定位
Nucleus, Cell membrane, CytoplasmAccession
O60716克隆号
SDT-R039抗体类型
Rabbit mAb反应种属 ?
Hu浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:500
Catenin δ-1 (P120 Catenin) belongs to the β-serial protein family .p-120 catenin (along with α, β and γ catenins) connects the transmembrane protein E-cadherin to the actin cytoskeleton in the cell cytoplasm. It expresses in various normal epithelial tissues (including thyroid, breast, stomach, duodenal, liver, pancreas, colon, kidney, prostate, testicles), various non -epithelial tissues (including myocardial cells, neurons, gel cells To. p-120 regulates the transformation of calcium adhesion protein on the surface of the cells, thereby determining the E-calcium adhesive protein levels used in the cells, and then plays an important role in the adhesion of the cells. Many studies have shown that p-120 irregularly expression or lack of disobedience in a variety of cancer cells indicates that p-120 play a role in cancer-suppressing genes.
免疫印迹
WB result of p120 Catenin Rabbit mAb Primary antibody: p120 Catenin Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Negative control: HL-60 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90~105 kDa
Observed MW: 90~105 kDa
Exposure time: 180sWB result of p120 Catenin Rabbit mAb Primary antibody: p120 Catenin Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90~105 kDa
Observed MW: 90~105 kDa
Exposure time: 180sWB result of p120 Catenin Rabbit mAb Primary antibody: p120 Catenin Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90~105 kDa
Observed MW: 90~105 kDa
Exposure time: 180s
免疫组化
IHC shows positive staining in paraffin-embedded human stomach.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human prostate.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human colon.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human tonsil.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human liver.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC shows positive staining in paraffin-embedded human colon cancer.
Anti-p120 Catenin antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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