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宿主来源
Rabbit抗原名称
AFP分子别名
Alpha-fetoprotein, Alpha-1-fetoprotein, HPAFP细胞定位
SecretedAccession
P02771克隆号
SDT-R031抗体类型
Rabbit mAb反应种属 ?
Hu, Ms纯化方式
Protein A浓度
1 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 WB 1:1000-1:2000 IHC-P 1:2000 ICC 1:60
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). Maternal AFP serum level is used to screen for Down syndrome, neural tube defects, and other chromosomal abnormalities. AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. It is thought to be the fetal analog of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms.
免疫印迹
WB result of AFP Rabbit mAb
Primary antibody: AFP Rabbit mAb at 1/2000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70kDa
Observed MW: 70kDaWB result of AFP Rabbit mAb
Primary antibody: AFP Rabbit mAb at 1/2000 dilution
Lane 1: mouse placenta lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70kDa
Observed MW: 70kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta.
Anti-AFP antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma.
Anti-AFP antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma.
Anti-AFP antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver.
Anti-AFP antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells. Anti-AFP antibody was used at 1/60 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (red).
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