Myoglobin Recombinant Rabbit mAb,PBS Only (SDT-097-10)

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货号： S0B2082P

Datasheet COA

价格：询价
规格：
1mg
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

Myoglobin
分子别名

Mb,MB
免疫原

Recombinant Protein
细胞定位

Cytoplasm
Accession

P02144
克隆号

SDT-097-10
抗体类型

Rabbit mAb
应用

ICFCM, IHC-P, WB, IF
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

1 mg/ml
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt, 4°C as supplied

稀释度

应用	稀释度
WB	1:500
IHC-P	1:1000
ICFCM	1:250
IF	1:1000

背景介绍

Myoglobin is a protein that's found in your striated muscles, which includes skeletal muscles (the muscles attached to your bones and tendons) and heart muscles. Its main function is to supply oxygen to the cells in your muscles (myocytes). All cells in your body need oxygen in order to function. Myoglobin is distantly related to hemoglobin. Compared to hemoglobin, myoglobin has a higher affinity for oxygen and does not have cooperative binding with oxygen like hemoglobin does. In humans, myoglobin is only found in the bloodstream after muscle injury.

验证数据
- Flow cytometric analysis of SK-BR-3 cells labelling Myoglobin antibody at 1/250 dilution (0.1ug)/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫印迹
- WB result of Myoglobin Rabbit mAb
  Primary antibody: Myoglobin Rabbit mAb at 1/500 dilution
  Lane 1: mouse brain lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Lane 3: mouse skeletal muscle lysate 20 µg
  Negative control: mouse brain lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 14 kDa
- WB result of Myoglobin Rabbit mAb
  Primary antibody: Myoglobin Rabbit mAb at 1/500 dilution
  Lane 1: rat brain lysate 20 µg
  Lane 2: rat heart lysate 20 µg
  Lane 3: rat skeletal muscle lysate 20 µg
  Negative control: rat brain lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 17 kDa
  Observed MW: 14 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human heart.
  Anti-Myoglobin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human skeletal muscle.
  Anti-Myoglobin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human liver.
  Anti-Myoglobin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse heart.
  Anti-Myoglobin antibody was used' at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat heart.
  Anti-Myoglobin antibody was used' at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human cardiac muscle. Anti-Myoglobin antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- Negative control: IF shows positive staining in paraffin-embedded human liver. Anti-Myoglobin antibody was used at 1/1000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.