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Rabbit抗原名称
TIA1分子别名
p40-TIA-1免疫原
N/A细胞定位
Cytoplasm, NucleusAccession
P31483克隆号
SDT-R026抗体类型
Rabbit mAb应用
FC, ICC, WB, IP反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:500 |
| WB | 1:1000 |
| IP | 1:25 |
| FC | 1:500 |
TIA1 or Tia1 cytotoxic granule-associated RNA binding protein is a 3'UTR mRNA binding protein that can bind the 5'TOP sequence of 5'TOP mRNAs. It is associated with programmed cell death (apoptosis) and regulates alternative splicing of the gene encoding the Fas receptor, an apoptosis-promoting protein. Under stress conditions, TIA1 localizes to cellular RNA-protein conglomerations called stress granules. It is encoded by the TIA1 gene.Mutations in the TIA1 gene have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, and Welander distal myopathy. It also plays a crucial role in the development of toxic oligomeric tau in Alzheimer's disease.
验证数据
Flow cytometric analysis of Jurkat cells labelling TIA1 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody.
免疫印迹
WB result of TIA1 Rabbit mAb
Primary antibody: TIA1 Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: MOLT-4 whole cell lysate 20 µg
Lane 3: Hela whole cell lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42~43 kDa
Observed MW: 42~43 kDa
Exposure time: 2.5s
免疫沉淀
TIA1 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating TIA1 in 0.4mg Molt-4 whole cell lysate.
Western blot was performed on the immunoprecipitate using TIA1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : Molt-4 whole cell lysate 10µg (input)
Lane 2 : TIA1 Rabbit mAb IP in Molt-4 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in Molt-4 whole cell lysate
Predicted MW: 42~43 kDa
Observed MW: 42~43 kDa
Exposure time: 60s
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti-TIA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
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