TIA1 Recombinant Rabbit mAb (S-R026)

p40-TIA-1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B2076

规格

25μl100μl500μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

TIA1
分子别名

p40-TIA-1
细胞定位

Cytoplasm, Nucleus
Accession

P31483
克隆号

SDT-R026
抗体类型

Rabbit mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

FCM

ICC ?

WB

IP
稀释度

应用稀释度

ICC 1:500
WB 1:1000
IP 1:25
FCM 1:500

应用	稀释度
ICC	1:500
WB	1:1000
IP	1:25
FCM	1:500

背景介绍

TIA1 or Tia1 cytotoxic granule-associated RNA binding protein is a 3'UTR mRNA binding protein that can bind the 5'TOP sequence of 5'TOP mRNAs. It is associated with programmed cell death (apoptosis) and regulates alternative splicing of the gene encoding the Fas receptor, an apoptosis-promoting protein. Under stress conditions, TIA1 localizes to cellular RNA-protein conglomerations called stress granules. It is encoded by the TIA1 gene.Mutations in the TIA1 gene have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, and Welander distal myopathy. It also plays a crucial role in the development of toxic oligomeric tau in Alzheimer's disease.

验证数据
- Flow cytometric analysis of Jurkat cells labelling TIA1 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody.
免疫印迹
- WB result of TIA1 Rabbit mAb
  Primary antibody: TIA1 Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: MOLT-4 whole cell lysate 20 µg
  Lane 3: Hela whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 42~43 kDa
  Observed MW: 42~43 kDa
  Exposure time: 2.5s
免疫沉淀
- TIA1 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating TIA1 in 0.4mg Molt-4 whole cell lysate.
  Western blot was performed on the immunoprecipitate using TIA1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1 : Molt-4 whole cell lysate 10µg (input)
  Lane 2 : TIA1 Rabbit mAb IP in Molt-4 whole cell lysate
  Lane 3 : Rabbit monoclonal IgG IP in Molt-4 whole cell lysate
  Predicted MW: 42~43 kDa
  Observed MW: 42~43 kDa
  Exposure time: 60s
免疫细胞化学
- ICC shows positive staining in Jurkat cells. Anti-TIA1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).