S-RMab^® Cytokeratin 7 Recombinant Rabbit mAb (SDT-121-17)

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货号： S0B2057

Datasheet COA

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产品规格

宿主来源

Rabbit
抗原名称

Cytokeratin 7
分子别名

Cytokeratin-7 (CK-7), Keratin-7 (K7), Sarcolectin, Type-II keratin Kb7, KRT7
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P08729
克隆号

SDT-121-17
抗体类型

Rabbit mAb
应用

IHC-P, FCM, ICC, WB, IP, IF
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IP	1:25
ICC	1:500
IHC-P	1:500
FCM	1:500
WB	1:1000
IF	1:200

背景介绍

Cytokeratin 7 is the intermediate silk protein mainly expressed in epithelial cells. The heterodine of keratin consists of a polymerization filament by an acid -keratin (or type I keratin, keratin 9 to 23) and alkaline keratin (or type II keratin, keratin 1 to 8). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium.It's enerally expressed (with some variation) in adenocarcinoma of lung, breast, thyroid, endometrium, cervix, ovary, salivary gland, upper GI tract, urothelial carcinoma, papillary renal cell carcinoma and Paget disease It's generally negative (with some variation) in colorectal carcinoma, Merkel cell carcinoma, hepatocellular carcinoma, prostatic adenocarcinoma, adrenocortical tumors and squamous cell carcinoma.

免疫印迹
- WB result of Cytokeratin 7 Rabbit mAb
  Primary antibody: Cytokeratin 7 Rabbit mAb at 1/1000 dilution
  Lane 1: MCF7 whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Lane 3: Hela whole cell lysate 20 µg
  Lane 4: SK-BR-3 whole cell lysate 20 µg
  Negative control: MCF7 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 40~55 kDa
  Observed MW: 40~55 kDa
  Exposure time: 40s
流式分析
- Flow cytometric analysis of MCF7 (Human monocytic leukemia monocyte, left) / HeLa (Human T cell leukemia T lymphocyte, right) cells labelling Cytokeratin 7 antibody at 1/500 dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 at 1/1000 dilution was used as the secondary antibody. Negative control: MCF7
免疫沉淀
- Cytokeratin 7 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Cytokeratin 7 in 0.4mg SK-BR-3 whole cell lysate. Western blot was performed on the immunoprecipitate using Cytokeratin 7 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: SK-BR-3 whole cell lysate 10µg (input)
  Lane 2: Cytokeratin 7 Rabbit mAb IP in SK-BR-3 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in SK-BR-3 whole cell lysate
  Predicted MW: 40~55 kDa
  Observed MW: 40~55 kDa
  Exposure time: 0.5s
免疫组化
- IHC shows positive staining in paraffin-embedded human breast cancer.
  Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast.
  Anti-Cytokeratin 7 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma cancer.
  Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer.
  Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human bladder cancer.
  Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta.
  Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control.IHC shows negative staining in paraffin-embedded human colon.Anti-Cytokeratin 7 antibody was used at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Cytokeratin 7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
- IF shows positive staining in paraffin-embedded human breast cancer. Anti-Cytokeratin 7 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Cytokeratin 7 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- Negative control: IF shows negative staining in paraffin-embedded human gastrointestinal stromal tumor. Anti-EpCAM antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.