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宿主来源
Rabbit抗原名称
LDL Receptor分子别名
Low-density lipoprotein receptor, LDLR免疫原
Synthetic Peptide细胞定位
Plasma Membrane, Lysosome, Endosome, Golgi ApparatusAccession
P01130克隆号
SDT-057-57抗体类型
Rabbit mAb应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:500 |
| IHC-P | 1:1000 |
The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors.Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis.LDLR is ubiquitously expressed and is a key receptor for maintaining cholesterol homeostasis in mammals. LDLR-mediated endocytosis is essential for lipoprotein and lipid metabolism.Impaired LDLR function by genetic mutations results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).
免疫印迹
WB result of LDL-receptor Rabbit mAb
Primary antibody: LDL-receptor Rabbit mAb at 1/500 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: HEK293 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: Hela whole cell lysate 20 µg
Low expression control: HEK293 whole cell lysatelow expression control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 140 kDa
Observed MW: 140 kDa
Exposure time: 120s
WB result of LDL-receptor Rabbit mAb
Primary antibody: LDL-receptor Rabbit mAb at 1/500 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: rat liver whole cell lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 140 kDa
Observed MW: 140 kDa
Exposure time: 180s
免疫组化
IHC shows positive staining in paraffin-embedded human liver.
Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer.
Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver.
Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach.
Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver.
Anti-LDL receptor antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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