S-RMab^® c-Met Recombinant Rabbit mAb (SDT-009-7H0L0)

浏览次数：24

货号： S0B2052

Datasheet COA

价格： ￥700
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

c-Met
分子别名

HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor (SF receptor), Tyrosine-protein kinase Met
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Membrane
Accession

P08581
克隆号

SDT-009-7H0L0
抗体类型

Rabbit mAb
抗体同种型

IgG
应用

IHC-P, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

2mg/ml
分子量

140kDa,170kDa
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
IHC-P	1:300
WB	1:40000

背景介绍

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. MET is a single pass tyrosine kinase receptor essential for embryonic development, organogenesis and wound healing. Hepatocyte growth factor/Scatter Factor (HGF/SF) and its splicing isoform (NK1, NK2) are the only known ligands of the MET receptor. MET is normally expressed by cells of epithelial origin, while expression of HGF/SF is restricted to cells of mesenchymal origin. When HGF/SF binds its cognate receptor MET it induces its dimerization through a not yet completely understood mechanism leading to its activation. Abnormal MET activation in cancer correlates with poor prognosis, where aberrantly active MET triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body. Both the overexpression of Met/HGFR, as well as its autocrine activation by co-expression of its hepatocyte growth factor ligand, have been implicated in oncogenesis.

免疫印迹
- WB result of c-Met Rabbit mAb
  Primary antibody: c-Met Rabbit mAb at 1/40000 dilution
  Lane 1: Hela lysate 20 µg
  Lane 2: HT-29 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 155 kDa
  Observed MW: 155 kDa
  Exposure time: 1s
免疫组化
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer.
  Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows weak staining in paraffin-embedded human ovarian cancer.
  Anti-c-Met antibody was used at 1/300 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.