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MCM3 Recombinant Rabbit mAb (SDT-048-10)

DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B2039
规格
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产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    MCM3

  • 分子别名

    DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Chromosome, Intracellular

  • Accession

    P25205

  • 克隆号

    SDT-048-10

  • 抗体类型

    Rabbit mAb

  • 反应种属 ?

    Hu

  • 预测反应种属
    (反应种属缩写表)

    Ms, Av, Or, Bv

  • 纯化方式

    Protein A

  • 浓度

    0.5mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB ,
    IP ,
    IF ?

  • 稀释度

    应用 稀释度
    ICFCM 1:500
    ICC 1:500
    IHC-P 1: 2000
    IP 1:25
    WB 1:2000
    IF 1:500
背景介绍

  • DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

  • 免疫印迹

    • WB result of MCM3 Rabbit mAb

      Primary antibody : MCM3 Rabbit mAb at 1/2000 dilution
      Lane 1: Hela whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: K562 whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 91 kDa
      Observed MW: 110 kDa
      Exposure time: 1.3 s

    • WB result of MCM3 Rabbit mAb

      Primary antibody : MCM3 Rabbit mAb at 1/2000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 91 kDa
      Observed MW: 110 kDa
      Exposure time: 4 s

  • 流式分析

    • Flow cytometric analysis of A431 cells labelling MCM3 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • MCM3 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating MCM3 in 0.4mg HeLa whole cell lysate. Western blot was performed on the immunoprecipitate using MCM3 Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HeLa whole cell lysate 10µg (input)
      Lane 2: MCM3 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 91 kDa
      Observed MW: 110 kDa
      Exposure time: 15s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human stomach.

      Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer.

      Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell cancer.

      Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen.

      Anti-MCM3 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-MCM3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human colon cancer . Anti-MCM3 antibody was used at 1/500 dilution (meganta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human cervical cancer . Anti-MCM3 antibody was used at 1/500 dilution (meganta) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 647) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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