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宿主来源
Rabbit抗原名称
CD23分子别名
BLAST-2, C-type lectin domain family 4 member J,Fc-epsilon-RII,Immunoglobulin E-binding factor, Lymphocyte IgE receptor, FCER2; CD23A,CLEC4J,FCE2, IGEBF免疫原
Synthetic PeptideAccession
P06734克隆号
SDT-028-67抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A研究领域
Immunology浓度
1 mg/ml分子量
37, 45kDa
标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 IHC-P 1:2000
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. CD23 is known to have a role of transportation in antibody feedback regulation. Antigens which enter the blood stream can be captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell lymphoma (CD23-negative). CD23 can also be demonstrated in germinal centre follicular dendritic cells using immunohistochemistry but is minimally expressed by benign germinal center B cells.
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-CD23 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-CD23 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell cancer.
Anti-CD23 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon.
Anti-CD23 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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