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宿主来源
Rabbit抗原名称
NSE分子别名
ENO2, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, neural enolase免疫原
Recombinant ProteinAccession
P09104克隆号
SDT-004-100抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A研究领域
Neuroscience浓度
1 mg/ml分子量
47kDa
标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 WB 1:500 IHC-P 1:1000
Neuron specific enolase (NSE) is a phosphopyruvate hydratase and is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. NSE as a serum biomarker has been widely used in diagnosis of various malignant tumors in clinical, and it has been found to be associated with melanoma, seminoma, renal cell carcinoma, Merkel cell tumor, carcinoid tumors, dysgerminomas, and immature teratomas, especially small cell lung cancer (SCLC).
免疫印迹
WB result of NSE Rabbit mAb
Primary antibody : NSE Rabbit mAb at 1/500 dilution
Lane 1 : Hela whole cell lysate 20 µg
Lane 2 : SK-BR-3 whole cell lysate 20 µg
Negative control: Hela whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 47 kDa
Exposure time: 120 seconds(This blot was developed with high sensitivity substrate)
免疫组化
IHC shows positive staining in paraffin-embedded human pancreas islet. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human lung adenocarcinoma(negative tissue). Anti-NSE antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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