S-RMab^® Keratin 14 Recombinant Rabbit mAb (SDT-023-12)

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货号： S0B2011

Datasheet COA

价格： ￥600
规格：
25μl100μl500μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

Keratin 14
分子别名

Cytokeratin-14,CK-14, KRT14
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P02533
克隆号

SDT-023-12
抗体类型

Rabbit mAb
抗体同种型

IgG
应用

ICFCM, IHC-P, ICC, WB
反应种属 ?

Hu, Ms
预测反应种属
(反应种属缩写表)

Rt
纯化方式

Protein A
研究领域

Signal Transduction
浓度

0.5mg/ml
分子量

51kDa
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
ICC	1:500
WB	1:5000-1:10000
IHC-P	1:4000
ICFCM	1:500

背景介绍

Keratin 14 is a member of the type I keratin family of intermediate filament proteins. Keratin 14 was the first type I keratin sequence determined. Keratin 14 is also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14). In humans it is encoded by the KRT14 gene. Keratin 14 is expressed in mitotically active basal layer cells, along with its partner keratin 5 (K5), and their expression is down-regulated as cells differentiate. Keratin 14 has been studied as a prognostic marker in breast cancer. Keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and has been reported useful in the identification of squamous cell carcinomas.

免疫印迹
- WB result of Keratin 14 Rabbit mAb
  Primary antibody : Anti-Keratin 14 antibody at 1/10000 dilution
  Lane 1 : A431 whole cell lysate 10 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  
  Predicted MW: 51 kDa
  Observed MW: 51 kDa
  Exposure time: 2 seconds
- WB result of Keratin 14 Rabbit mAb
  Primary antibody : Anti-Keratin 14 antibody at 1/5000 dilution
  Lane 1 : mouse skin lysate 10 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  
  Predicted MW: 51 kDa
  Observed MW: 51 kDa
  Exposure time: 2 seconds
流式分析
- Flow cytometric analysis of A431 cells labelling Keratin-14 antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows membrane staining in paraffin-embedded human tonsil squamous epithelium.
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded human skin.
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded basal epithelium of human breast.
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded human esophagus. Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows negative staining in paraffin-embedded human liver (negative tissue).
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded human cervix cancer. Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded human lung squamous cell cancer.
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows membrane staining in paraffin-embedded mouse skin.
  Anti-Keratin 14 antibody was used at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  Counterstained with hematoxylin.
  Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-Keratin 14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).