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宿主来源
Rabbit抗原名称
MCM2分子别名
BM28, CCNL1, CDCL1, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28免疫原
Synthetic Peptide细胞定位
NucleusAccession
P49736克隆号
SDT-018-68-2抗体类型
Rabbit mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A研究领域
Epigenetics浓度
1 mg/ml分子量
125kDa
标记
Unconjugated性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000-1:10000 Hu IHC-P 1:1000 Hu, Ms ICFCM 1:500 Hu ICC 1:500 Hu
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM2 forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. MCM2 is phosphorylated, and thus regulated by protein kinases CDC2 and CDC7.
免疫印迹
WB result of MCM2 Rabbit mAb
Primary antibody : MCM2 Rabbit mAb at 1:2000 dilution
Lane 1 : Hela whole cell lysate 20 µg
Lane 2 : Ramos whole cell lysate 20 µg
Lane 3 : Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 125 kDa
Exposure time: 0.5 seconds
流式分析
Flow cytometric analysis of Jurkat cells labelling MCM2 antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC show nucleus staining in paraffin-embedded human tonsil. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human bladder cancer. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human breast cancer. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human gastric cancer. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human lung adenocarcinoma. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human pancreatic cancer. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded human thyroid cancer. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.IHC show nucleus staining in paraffin-embedded mouse spleen. Anti-MCM2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-MCM2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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