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宿主来源
Rabbit抗原名称
Bcl-2免疫原
Synthetic PeptideAccession
P10415克隆号
SDT-005-24抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A研究领域
Apoptosis浓度
1 mg/ml分子量
26kDa
标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 IHC-P 1:500-1:2000 Hu WB 1:1000-1:5000 Hu
Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis. It was the first apoptosis regulator identified in any organism. Bcl-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.
免疫印迹
WB result of anti-Bcl-2 antibody
Primary antibody : Anti-Bcl2 antibody at 1/5000 dilution
Lane 1 : Daudi whole cell lysate 20 µg
Lane 2 : Jurkat whole cell lysate 20 µg
Lane 3 : THP-1 whole cell lysate 20 µg
Lane 4 : MCF7 whole cell lysate 20 µg
Lane 5 : Hela whole cell lysate 20 µg
Negative control: Daudi whole cell lysateSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-Bcl2 antibody was used at 1:500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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