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宿主来源
Mouse抗原名称
Lamin B1分子别名
Lamin-B1; LMN2; LMNB; LMNB1细胞定位
Nucleus laminaAccession
P20700克隆号
S-4865抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, PC-3, HepG2, HEK-293, Jurkat, K562, NIH/3T3, RAW264.7, 4T1, mouse spleen, PC-12, C6, rat spleen预测反应种属
(反应种属缩写表)Rb, CDV, Ck, Zf, Bv, Hm纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:20000-1:100000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:2000 Hu
Lamin B1 is a crucial type V intermediate filament protein and a major structural component of the nuclear lamina, a dense fibrillar network located on the inner side of the inner nuclear membrane in eukaryotic cells. Unlike its counterparts Lamin A and C, which are developmentally regulated and often absent in early embryonic stages or certain cell types, Lamin B1 is ubiquitously expressed and considered essential for cell viability, playing indispensable roles in maintaining nuclear shape, mechanical stability, and the spatial organization of chromatin. It serves as a scaffold for anchoring chromatin to the nuclear periphery, thereby influencing gene regulation, DNA replication, and repair processes, while also interacting with various integral membrane proteins to ensure proper nuclear envelope integrity during interphase and facilitating the disassembly and reassembly of the nuclear envelope during mitosis; consequently, dysregulation or mutations in the LMNB1 gene have been linked to severe neurological disorders, such as adult-onset autosomal dominant leukodystrophy, highlighting its critical importance in both cellular architecture and human health.
免疫印迹
WB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDaWB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: 4T1 whole cell lysate 20 µg
Lane 4: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDaWB result of Lamin B1 Recombinant Mouse mAb
Primary antibody: Lamin B1 Recombinant Mouse mAb at 1/100000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 66 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Lamin B1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Lamin B1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Lamin B1 in tumor tissues.
Expression of Lamin B1 in human tissues.
Expression of Lamin B1 in mouse & rat tissues.
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