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Rabbit抗原名称
Stat6分子别名
Signal transducer and activator of transcription 6; IL-4 Stat; STAT6免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P42226克隆号
SDT-337-32抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A浓度
1 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICFCM
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:200 Hu IF 1:200 Hu ICFCM 1:500
Stat6 protein, which stands for Signal Transducer and Activator of Transcription 6, is a crucial component in the immune system. It is a member of the Stat family of transcription factors that play key roles in transmitting signals from cytokine receptors to the nucleus. When activated by cytokines like IL-4 and IL-13, Stat6 translocates to the nucleus and regulates the expression of numerous genes involved in immune responses, such as those related to the differentiation of T helper 2 cells and the production of immunoglobulin E. This protein is essential for mounting effective immune responses against certain pathogens and also has implications in various immune-related diseases, including asthma and allergic reactions.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (meganta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human colon. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human kidney. Anti-Stat6 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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