分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
BrdU细胞定位
Nucleus克隆号
S-R639抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Species Independent阳性样本
Hela cells treated with BrdU纯化方式
Protein G浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:500 Hu
BrdU (5-bromo-2'-deoxyuridine) is a thymidine analog that can be incorporated into newly synthesized DNA strands during DNA replication, used to detect cell proliferation. BrdU antibodies are monoclonal or polyclonal antibodies that specifically recognize BrdU-labeled DNA and are widely used in experiments such as immunohistochemistry and flow cytometry to visualize or quantitatively analyze proliferating cells.
免疫细胞化学
ICC analysis of Hela cells treated with BrdU (10uM for 24 hours) (top panel) and untreated THP-1 cells (below panel). The cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. The cells were then permeabilized with 0.1% PBS-Triton X-100. Anti- BrdU (FITC Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
评论(0)