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Rabbit抗原名称
GAPDH分子别名
Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH免疫原
Synthetic Peptide细胞定位
Membrane, Cytoplasm, NucleusAccession
P04406, P16858克隆号
S-240-147抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC反应种属 ?
Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:50-1:100 | Ms, Rt |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules [PMID: 17072346]. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- GAPDH (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti- GAPDH (Alexa Fluor® 488 Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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