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宿主来源
Armenian hamster抗原名称
CD27分子别名
CD27 antigen; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; TNFRSF7Accession
P26842, P41272, Q501W2克隆号
S-R620抗体类型
Recombinant mAb应用
FCM反应种属 ?
Hu, Ms, Rt阳性样本
Human PBMC, Mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Hu, Ms |
CD27 is a transmembrane protein that belongs to the tumor necrosis factor receptor superfamily (TNFRSF) and is primarily expressed on T cells, B cells, and natural killer (NK) cells. It activates NF-κB and JNK signaling pathways by binding to its ligand, CD70, thereby promoting the activation, proliferation, and differentiation of T cells. CD27 plays a crucial role in immune regulation, acting as an important co-stimulatory receptor for T and B cells, and is involved in modulating immune responses and the formation of memory cells. Additionally, CD27 is being studied as a potential target for anti-tumor immunotherapy.
流式分析
Flow cytometric analysis of CD27 expression on human peripheral blood. Human peripheral blood was stained with Brilliant Violet 421™ Mouse Anti-Human CD19 antibody and either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat/Human CD27 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software. Flow cytometric analysis of CD27 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with PE/Cy7 Rat Anti-Mouse CD19 antibody and either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat/Human CD27 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software. Flow cytometric analysis of CD27 expression on SD Rat splenocytes. SD Rat splenocytes were stained with either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat/Human CD27 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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