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宿主来源
Mouse抗原名称
CD90分子别名
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen, THY1细胞定位
Cell membraneAccession
P04216克隆号
5E10抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
RhMk, CyMk, Hu阳性样本
Jurkat纯化方式
Protein G浓度
0.02mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5 μl per million cells in 100μl volume Hu
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
流式分析
Flow cytometric analysis of Human CD90 expression on Jurkat cells. Cells from the Jurkat (Human T cell leukemia T lymphocyte, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with PerCP-Cy5.5 Mouse IgG1 Isotype Control (Black line histogram) and SDT PerCP-Cy5.5 Mouse Anti-Human CD90 antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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