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宿主来源
Rat抗原名称
Mouse CD335分子别名
Natural cytotoxicity triggering receptor 1; Activating receptor 1 (mAR-1); Lymphocyte antigen 94; Natural killer cell p46-related protein (NK-p46; NKp46; mNKp46); Ncr1; Ly94细胞定位
Cell membraneAccession
Q8C567克隆号
S-R613抗体类型
Rat mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Ms |
CD335, also known as NKp46, is a 46kDa natural cytotoxicity receptor that belongs to the immunoglobulin superfamily. It is expressed on resting and activated NK cells, ILC1, a subset of NCR+ ILC3 in the mucosa, and a small subset of T lymphocytes. CD335 plays a crucial role in the lysis of autologous, allogeneic, or xenogeneic cells by NK cells and is highly conserved in mammals.
流式分析
Flow cytometric analysis of Mouse CD335 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse NK1.1 antibody and either FITC Rat IgG2a, κ Isotype Control (Left panel) or SDT FITC Rat Anti-Mouse CD335 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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