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宿主来源
Rat抗原名称
Mouse CD14分子别名
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein细胞定位
Cell membraneAccession
P10810克隆号
S-R609抗体类型
Rat mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Ms阳性样本
RAW 264.7纯化方式
Protein G浓度
0.05mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 5 μl per million cells in 100μl volume | Ms |
CD14, also known as the LPS receptor, is a surface antigen belonging to the family of cell surface glycoproteins. It exists in two forms: membrane-bound CD14 (mCD14), which is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) tail, and soluble CD14 (sCD14), found in the plasma. CD14 is primarily expressed on monocytes, macrophages, and dendritic cells, and plays a critical role in the immune system by recognizing and binding to lipopolysaccharide (LPS) from Gram-negative bacteria, leading to cellular activation and the initiation of inflammatory responses.
流式分析
Flow cytometric analysis of Mouse CD14 expression on RAW 264.7 Cells from the RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) or Neuro-2a (Mouse neuroblastoma neuroblast, Left) was stained with FITC Rat IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Rat Anti-Mouse CD14 Antibody (Red line histogram) at 0.25 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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