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宿主来源
Mouse抗原名称
CD28分子别名
T-cell-specific surface glycoprotein CD28; TP44细胞定位
Cell membraneAccession
P10747克隆号
CD28.2抗体类型
Mouse mAb抗体同种型
IgG1同型对照
S0B1529反应种属 ?
RhMk, CyMk, Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2-8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu, RhMk, CyMk
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.
流式分析
Flow cytometric analysis of human CD28 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 antibody and either FITC Mouse IgG1 Isotype Control (Left panel) or SDT FITC Mouse Anti-Human CD28 Antibody (Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of CD28 expression on Rhesus monkey peripheral blood cells. Rhesus macaque peripheral blood cells were stained with FITC Mouse IgG1, κ Isotype Control (left panel) or SDT FITC Mouse Anti- Human CD28 Antibody (right panel) at 1 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD28 expression on Cynomolgus monkey peripheral blood cells. Cynomolgus macaque peripheral blood cells were stained with FITC Mouse IgG1, κ Isotype Control (left panel) or SDT FITC Mouse Anti- Human CD28 Antibody (right panel) at 1 μg/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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