分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
LAMP1分子别名
Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; CD107a免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P11279克隆号
SDT-405-39抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICFCM
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:500 Hu ICFCM 1:800 Hu
Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The LAMP-1 glycoprotein is a type I transmembrane protein [PMID: 16973206] which is expressed at high or medium levels in at least 76 different normal tissue cell types. It resides primarily across lysosomal membranes [PMID: 2584229], and functions to provide selectins with carbohydrate ligands. LAMP-1 has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells, and may also play a role in tumor cell differentiation and metastasis.
流式分析
Flow cytometric analysis of LAMP1 expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Alexa Fluor® 647 Rabbit IgG Isotype Control (Black line histogram) and SDT LAMP1 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- LAMP1 (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
评论(0)