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Rabbit抗原名称
p27分子别名
Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cdkn1b; KIP-1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P46414克隆号
SDT-361-310抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Fg, Ct纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 推荐种属 IF 1:50 Hu
p27 KIP1 is a member of the Cyclin Inhibitory Protein/Kinase Inhibitory Protein (CIP/KIP) family of cyclin-dependent kinase (Cdk) inhibitors. It plays roles in all phases of the cell division cycle. Its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes enforces the G1 restriction point. Thus, important cellular functions as the cell division cycle, respiration, RNA processing, translation, and cell adhesion, are under p27 KIP1 regulation. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung.
免疫荧光
IF shows positive staining in paraffin-embedded human bladder cancer. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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