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宿主来源
Rabbit抗原名称
His tag免疫原
Synthetic Peptide克隆号
S-1398-10抗体类型
Recombinant mAb抗体同种型
IgG应用
FCM, ICC反应种属 ?
Species Independent纯化方式
Protein A浓度
2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Species independent |
| FCM | 1:800 | Species independent |
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
流式分析
Myc-His-tag transfected 293T (Human embryonic kidney epithelial cell, Right panel) or 293T (Left panel) was stained with either FITC Rabbit IgG Isotype Control (Black line histogram) or SDT His tag Recombinant Rabbit mAb (FITC Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
ICC shows positive staining in Histone H3-TAP-myc-his Tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- His tag (FITC Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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