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宿主来源
Rat抗原名称
Mouse Ly-6G/Ly-6C (Gr-1)分子别名
Lymphocyte antigen 6G; Ly-6G.1细胞定位
Cell membraneAccession
P35461克隆号
S-R425抗体类型
Rat mAb抗体同种型
IgG2b,k应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用 | 稀释度 | 推荐种属 |
---|---|---|
FCM | 0.1μg per million cells in 100μl volume | Ms |
Ly-6G protein is a GPI (glycosylphosphatidylinositol)-anchored protein. Ly-6G is primarily expressed on monocytes, neutrophils, and T cells. It plays a crucial role in inhibiting anti-tumor efficacy and T-cell responses. Due to its specific expression on neutrophils, Ly-6G has been widely used as a surface marker in flow cytometry, cell sorting, and immune phenotyping experiments. Unique expression of Ly-6G in bone marrow indicates its involvement in hematopoietic microenvironment, potentially regulating the development or function of immune cells within the bone marrow niche.
流式分析
Flow cytometric analysis of Mouse Ly-6G/Ly-6C (Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b antibody and SDT Biotin Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody at 0.1 μg/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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